Gene expression profiling of Listeria monocytogenes strain F2365 during growth in ultrahigh-temperature-processed skim milk.
Identifieur interne : 002981 ( Main/Exploration ); précédent : 002980; suivant : 002982Gene expression profiling of Listeria monocytogenes strain F2365 during growth in ultrahigh-temperature-processed skim milk.
Auteurs : Yanhong Liu [États-Unis] ; Amy ReamSource :
- Applied and environmental microbiology [ 1098-5336 ] ; 2008.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Analyse de profil d'expression de gènes, Animaux, Lait (microbiologie), Listeria monocytogenes (croissance et développement), Listeria monocytogenes (physiologie), Protéines bactériennes (génétique), RT-PCR, Régulation de l'expression des gènes bactériens, Séquençage par oligonucléotides en batterie, Température élevée.
- MESH :
- croissance et développement : Listeria monocytogenes.
- génétique : Protéines bactériennes.
- microbiologie : Lait.
- physiologie : Listeria monocytogenes.
- Adaptation physiologique, Analyse de profil d'expression de gènes, Animaux, RT-PCR, Régulation de l'expression des gènes bactériens, Séquençage par oligonucléotides en batterie, Température élevée.
English descriptors
- KwdEn :
- Adaptation, Physiological, Animals, Bacterial Proteins (genetics), Gene Expression Profiling, Gene Expression Regulation, Bacterial, Hot Temperature, Listeria monocytogenes (growth & development), Listeria monocytogenes (physiology), Milk (microbiology), Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
- MESH :
- chemical , genetics : Bacterial Proteins.
- growth & development : Listeria monocytogenes.
- microbiology : Milk.
- physiology : Listeria monocytogenes.
- Adaptation, Physiological, Animals, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Hot Temperature, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
To study how Listeria monocytogenes survives and grows in ultrahigh-temperature-processed (UHT) skim milk, microarray technology was used to monitor the gene expression profiles of strain F2365 in UHT skim milk. Total RNA was isolated from strain F2365 in UHT skim milk after 24 h of growth at 4 degrees C, labeled with fluorescent dyes, and hybridized to "custom-made" commercial oligonucleotide (35-mers) microarray chips containing the whole genome of L. monocytogenes strain F2365. Compared to L. monocytogenes grown in brain heart infusion (BHI) broth for 24 h at 4 degrees C, 26 genes were upregulated (more-than-twofold increase) in UHT skim milk, whereas 14 genes were downregulated (less-than-twofold decrease). The upregulated genes included genes encoding transport and binding proteins, transcriptional regulators, proteins in amino acid biosynthesis and energy metabolism, protein synthesis, cell division, and hypothetical proteins. The downregulated genes included genes that encode transport and binding proteins, protein synthesis, cellular processes, cell envelope, energy metabolism, a transcriptional regulator, and an unknown protein. The gene expression changes determined by microarray assays were confirmed by real-time reverse transcriptase PCR analyses. Furthermore, cells grown in UHT skim milk displayed the same sensitivity to hydrogen peroxide as cells grown in BHI, demonstrating that the elevated levels of expression of genes encoding manganese transporter complexes in UHT skim milk did not result in changes in the oxidative stress sensitivity. To our knowledge, this report represents a novel study of global transcriptional gene expression profiling of L. monocytogenes in a liquid food.
DOI: 10.1128/AEM.00356-08
PubMed: 18806004
Affiliations:
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Yanhong.Liu@ars.usda.gov</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Microbial Food Safety Research Unit, Eastern Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19038</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Ream, Amy" sort="Ream, Amy" uniqKey="Ream A" first="Amy" last="Ream">Amy Ream</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="eISSN">1098-5336</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Bacterial Proteins (genetics)</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Bacterial</term><term>Hot Temperature</term><term>Listeria monocytogenes (growth & development)</term><term>Listeria monocytogenes (physiology)</term><term>Milk (microbiology)</term><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique</term><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>Lait (microbiologie)</term><term>Listeria monocytogenes (croissance et développement)</term><term>Listeria monocytogenes (physiologie)</term><term>Protéines bactériennes (génétique)</term><term>RT-PCR</term><term>Régulation de l'expression des gènes bactériens</term><term>Séquençage par oligonucléotides en batterie</term><term>Température élevée</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lait</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Milk</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Bacterial</term><term>Hot Temperature</term><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>RT-PCR</term><term>Régulation de l'expression des gènes bactériens</term><term>Séquençage par oligonucléotides en batterie</term><term>Température élevée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To study how Listeria monocytogenes survives and grows in ultrahigh-temperature-processed (UHT) skim milk, microarray technology was used to monitor the gene expression profiles of strain F2365 in UHT skim milk. Total RNA was isolated from strain F2365 in UHT skim milk after 24 h of growth at 4 degrees C, labeled with fluorescent dyes, and hybridized to "custom-made" commercial oligonucleotide (35-mers) microarray chips containing the whole genome of L. monocytogenes strain F2365. Compared to L. monocytogenes grown in brain heart infusion (BHI) broth for 24 h at 4 degrees C, 26 genes were upregulated (more-than-twofold increase) in UHT skim milk, whereas 14 genes were downregulated (less-than-twofold decrease). The upregulated genes included genes encoding transport and binding proteins, transcriptional regulators, proteins in amino acid biosynthesis and energy metabolism, protein synthesis, cell division, and hypothetical proteins. The downregulated genes included genes that encode transport and binding proteins, protein synthesis, cellular processes, cell envelope, energy metabolism, a transcriptional regulator, and an unknown protein. The gene expression changes determined by microarray assays were confirmed by real-time reverse transcriptase PCR analyses. Furthermore, cells grown in UHT skim milk displayed the same sensitivity to hydrogen peroxide as cells grown in BHI, demonstrating that the elevated levels of expression of genes encoding manganese transporter complexes in UHT skim milk did not result in changes in the oxidative stress sensitivity. To our knowledge, this report represents a novel study of global transcriptional gene expression profiling of L. monocytogenes in a liquid food.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><noCountry><name sortKey="Ream, Amy" sort="Ream, Amy" uniqKey="Ream A" first="Amy" last="Ream">Amy Ream</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Liu, Yanhong" sort="Liu, Yanhong" uniqKey="Liu Y" first="Yanhong" last="Liu">Yanhong Liu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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